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Team:Paris/Production overview
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== Overview == | == Overview == | ||
| - | --- | + | ---- |
'''Constructions''' | '''Constructions''' | ||
| - | --- | + | ---- |
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| + | *The creation of OMV's | ||
| + | |||
| + | To create the vesicules, we started with a first simple construction only containing the unfunctional TolR II biobrick placed donwstream a pTetR promoter. With the presence of arabinose in the medium, pBad is activated and TetR, a repressor of the pTetR pomoter in created. The addition of of tetracycline or its analog, aTc, in th emedium inhibits the repression thus allowing the creation of vesicules. | ||
| + | |||
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| + | *The delay | ||
Revision as of 10:14, 30 July 2009
Overview
Constructions
- The creation of OMV's
To create the vesicules, we started with a first simple construction only containing the unfunctional TolR II biobrick placed donwstream a pTetR promoter. With the presence of arabinose in the medium, pBad is activated and TetR, a repressor of the pTetR pomoter in created. The addition of of tetracycline or its analog, aTc, in th emedium inhibits the repression thus allowing the creation of vesicules.
- The delay
