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Team:Paris/Production overview
From 2009.igem.org
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| - | For further precisions on the biobricks used as well as the plasmid backbones, please visit our parts design page [ | + | For further precisions on the biobricks used as well as the plasmid backbones, please visit our parts design page [Team:Paris/Production_design Link title] |
*'''''The Delay''''' | *'''''The Delay''''' | ||
Revision as of 10:26, 30 July 2009
Overview
Constructions
- Creation of OMVs
To create the vesicules, we started with a first simple construction only containing the unfunctional TolR II biobrick placed donwstream a pTetR promoter. With the presence of arabinose in the medium, pBad is activated and TetR, a repressor of the pTetR pomoter in created. The addition of of tetracycline or its analog, aTc, in the medium inhibits the repression thus allowing the creation of vesicules.
For further precisions on the biobricks used as well as the plasmid backbones, please visit our parts design page [Team:Paris/Production_design Link title]
- The Delay
