Team:KULeuven/Freezer

From 2009.igem.org

(Difference between revisions)
(Blue light receptor)
(Blue light receptor)
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| {{kulpart|BBa_K238000}}||/||BLR promoter region||4 microcentrifuge tubes||||||july 30
| {{kulpart|BBa_K238000}}||/||BLR promoter region||4 microcentrifuge tubes||||||july 30
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|-
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| {{iGempart|BBa_pSB3K3-P1010}}||/||kanamycine pasmide backbone|| taken from plates || 13μl ||||july 29
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| {{iGempart|BBa_J23101}}||/||constitutive promotor||4 microcentrifuge tubes|| 13μl ||||july 30
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Revision as of 13:59, 11 August 2009

Contents

Blue light receptor

part plasmid description status μl left concentration date
MC4100 / strain of E.coli july 24
2171 / forward primer for 90µl 20μM july 24
2172 / reverse primer for 90µl 20μM july 24
2171 / forward primer for 100μM july 24
2172 / reverse primer for 100μM july 24
2260 / forward primer for 45µl 20μM aug 10
2261 / reverse primer for 45µl 20μM aug 10
2260 / forward primer for 100μM aug 10
2261 / reverse primer for 100μM aug 10
pSB1A2GFP + rbs + terminatorrestricted with EcoRI and XbaIjuly 29
/BLR promoter regionrestricted with EcoRIjuly 29
/BLR promoter region4 microcentrifuge tubesjuly 30
Template:IGempart/kanamycine pasmide backbone taken from plates 13μl july 29
Template:IGempart/constitutive promotor4 microcentrifuge tubes 13μl july 30

Key/antikey/lock

part plasmid description status μl left concentration date
/GFP with LVA tagpurifiedjuly 28
/tetR promoter purifiedjuly 28

vanillin synthesis

part plasmid description status μl left concentration date
pSB1A2ech + rbspurified plasmid108,8 ng/µlAugust 4
pSB1A2fcs + rbspurified plasmid113,9 ng/µlAugust 4
pSB1A2Sam5 + rbsreceived from EdinburghAugust 3
pSB1A2Sam5 + rbs, PstI site removedpurified plasmid101,3 ng/µlAugust 5
pSB1A2Sam8 + rbspurified plasmid95,7 ng/µlAugust 5
pSB1A2Sam8purified plasmid81,5 ng/µlAugust 5
pSB1A2COMTpurified plasmid122,9 ng/µlAugust 5
pSB1A2COMT + rbsplasmid from registryAugust 6
pSB1A2ech + rbspurified digest16,9 ng/µlAugust 6
fcs + rbspurified digest16,6 ng/µlAugust 6
pSB1A2sam5 + rbspurified digest3,1 ng/µlAugust 7
sam8 + rbspurified digest3,4 ng/µl August 7

vanillin receptor

part plasmid description status μl left concentration date
pSB1A2ech + rbspurified plasmid108,8 ng/µlAugust 4
pSB1A2fcs + rbspurified plasmid113,9 ng/µlAugust 4
pSB1A2Sam5 + rbsreceived from EdinburghAugust 3
pSB1A2Sam5 + rbs, PstI site removedpurified plasmid101,3 ng/µlAugust 5

miscellaneous

part plasmid description status μl left concentration date
dNTP mixATP, GTP, TTP, CTP9410μMaug 10
pSB1A2medium constitutieve promoterjuly 23
ampicilline