Team:Groningen/Notebook/8 September 2009

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Revision as of 14:46, 7 September 2009

Wet

GVP Cluster

→ TODO Check transfer of pArsR-GVP, pZntR-GVP, pCueO-GVP, and pLacI-GVP from J61035 and pSB1A2 to pSB2K3. (preculture growth, isolation, and restriction (the colonies all grew on kanamycin plates with IPTG, this is the first selection, because the parts come from an ampicillin plasmid))

→ DONE Cut out GVP cluster from biobrick vector (can be used for fragment formation)

→ TODO Create fragments for 400bp repeat removal from the gvpL gene. (restriction, gel purification)

→ TODO Perform ligation of all fragments into pSB1AC3 vector. (the pSB1AC3 vector has been the most succesfull vector to use in assemblies)

→ TODO Grow L-GVP, M-GVP, and pLacI-GVP (with IPTG) on plates for test of buoancy in Saline solution. (as control use pNL29 with IPTG, and J61002-pCueO)(precultures, plates)

→ TODO Check Saline solutions and take pictures!

Transporters

Metal Accumulation

Vectors

Dry