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Template:Team:KULeuven/25 August 2009/BlueLightReceptor
From 2009.igem.org
(Difference between revisions)
| Line 10: | Line 10: | ||
*Gel electrophoresis and extraction of the RD of {{kulpart|pSB3K3}} | *Gel electrophoresis and extraction of the RD of {{kulpart|pSB3K3}} | ||
--> failed. There was no signal at all | --> failed. There was no signal at all | ||
| - | *New colonies were | + | *New colonies were ented in liquid culture. |
*Ligation C performed: {{kulpart|BBa_J23101}} (A) + {{kulpart|BBa_E0240}} | *Ligation C performed: {{kulpart|BBa_J23101}} (A) + {{kulpart|BBa_E0240}} | ||
{| border ="1" align="center" | {| border ="1" align="center" | ||
Latest revision as of 12:30, 14 September 2009
- Miniprepping the liquid cultures with
| Part | concentration (ng/μl) | 260/280 λ |
|---|---|---|
| (1) | 27,6 | 1,91 |
| (2) | 47,7 | 2,08 |
- Restriction digest of with EcoRI and PstI
- Gel electrophoresis and extraction of the RD of
--> failed. There was no signal at all
- New colonies were ented in liquid culture.
- Ligation C performed: (A) +
| Vector | insert |
| + | |
| 30ng -> 1,4μl | 40,8ng -> 2μl |
