Team:Groningen/Modelling/Characterization

From 2009.igem.org

(Difference between revisions)
m (Fix for AsT on x-axis)
m
Line 202: Line 202:
       cost += Math.sqrt(curcost/n); // Compute the square root of the average of the squares (RMS)
       cost += Math.sqrt(curcost/n); // Compute the square root of the average of the squares (RMS)
       weight++;
       weight++;
 +
 +
      // Set last solution
 +
      e[i].solution = {'cost':curcost, 'xt':xt};
     } else if (e[i].time==Infinity) { // Vary AsT, with equilibrium
     } else if (e[i].time==Infinity) { // Vary AsT, with equilibrium
       var avgv = {};
       var avgv = {};
Line 224: Line 227:
       weight++;
       weight++;
     }
     }
-
 
-
    // Set last solution
 
-
    e[i].solution = {'cost':curcost, 'xt':xt};
 
   }
   }
   return cost/weight; // Take the average of the RMS values for all graphs, making it "easier" to disregard certain experiments in favour of the rest.
   return cost/weight; // Take the average of the RMS values for all graphs, making it "easier" to disregard certain experiments in favour of the rest.
Line 329: Line 329:
   document.getElementById('Meng2004Graph').refresh();
   document.getElementById('Meng2004Graph').refresh();
   document.getElementById('Singh2008Graph').refresh();
   document.getElementById('Singh2008Graph').refresh();
 +
  document.getElementById('Kostal2004fig3AGraph').refresh();
}
}
Line 440: Line 441:
|{{graph|Team:Groningen/Graphs/Characterization/GlpF|id=Meng2004Graph}}
|{{graph|Team:Groningen/Graphs/Characterization/GlpF|id=Meng2004Graph}}
|{{graph|Team:Groningen/Graphs/Characterization/Singh2008|id=Singh2008Graph}}
|{{graph|Team:Groningen/Graphs/Characterization/Singh2008|id=Singh2008Graph}}
 +
|-
 +
|{{graph|Team:Groningen/Graphs/Characterization/Kostal2004fig3A|id=Kostal2004fig3AGraph}}
|}
|}
 +
<!-- Don't forget to update the refreshGraphs function above! -->

Revision as of 12:32, 6 October 2009

[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]


TODO: Talk about the devices we have and in what way we want to characterize them.

Uptake measurements

Sampling scheme
Time (min)
0 10 20 40 60
As(III)exT(0)
(µM)
0 x
10 x x x x x
20 x
50 x
100 x

To efficiently look at both time and concentration dependent processes we took samples as in the table on the right. Below we list all results, which have been used for fitting all necessary parameters.

TODO: List results. Take conversion from nmol/mg and mg/ml to µM and Vc/Vs into account.

best cur gradient solved
v5/K5
v5
K5
k8/K7
k8
K7
tauBbeta4
tauB
beta4
tauR
beta1
E

Loading graph...
Loading graph...
Loading graph...