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| | <ul> | | <ul> |
| - | <li>drei Oligo's: zwei für die Bindung der Monomere und eines zur Erzeugung der dsDNA an die FokI binden kann.</li> | + | <li>three oligo's: two for binding the monomers and one to create a dsDNA where FokI can bind.</li> |
| - | <li>zwei Oligo's: eines zur Bindung des einen Monomers, das zweite zur Bindung des zweiten Monomers und zur Ausbildung der dsDNA (ein Monomer müsste hierfür inaktiviert werden).</li> | + | <li>two oligo's: one for binding one monomer, the second for binding the second monomer and to create dsDNA (one monomer has to be inactivated).</li> |
| - | <li>ein Oligo: beide Bindestellen der Monomere auf einem Oligo. Auch hier müsste ein Monomer inaktiviert sein.</li> | + | <li>one oligo: both binding sites of the monomers on one oligo (also one monomer has to be inactivated).</li> |
| | + | <br> |
| | + | The 196 C-terminale aminoacids of FokI were used from other groups (see reference 1). Primersequences are also provided in the publication.<br> |
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| - | Es wurde die 196 C-terminalen Aminosäuren von FokI verwendet. Primersequenzen sind vorhanden.<br>
| + | Reference 1: Freiburg09_FokI_Zn-Finger_1996.pdf |
| - | | + | <br><br> |
| - | <a href="Freiburg09_FokI_Zn-Finger_1996.pdf"</a><br><br><br>
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| | <table> | | <table> |
| | <tr> | | <tr> |
| - | <td><img src="https://static.igem.org/mediawiki/2009/9/99/Freiburg09_huebsche_fokI.jpg" name="Figure 1: FokI-Model, ribbon-style. DNA Strand is bound by two FOKI" width="400" height="400" alt="FokI bound to dsDNA" /></td> | + | <td><img src="https://static.igem.org/mediawiki/2009/9/99/Freiburg09_huebsche_fokI.jpg" name="Figure 1: FokI-Model, ribbon-style. DNA Strand is bound by two FOKI" width="300" height="300" alt="FokI bound to dsDNA" /></td> |
| | + | |
| | + | <td><img src="https://static.igem.org/mediawiki/2009/2/23/Freiburg09_huebsche_fokI_surface_render.jpg" name="Figure 2: FokI-Model surface rendering. DNA Strand is bound by two FOKI" width="300" height="300" /></td> |
| | + | </tr> |
| | | | |
| - | <td>[[Image:freiburg09_huebsche fokI_surface_render.jpg|none|thumb|Figure 2: FokI-Model surface rendering. DNA Strand is bound by two FOKI width="400" height="400"></td></tr>
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| | <tr> | | <tr> |
| | + | <td><img src="https://static.igem.org/mediawiki/2009/6/6a/Freiburg09_fokI_3oligos.JPG" name="Schema 3: 2Oligos, both with active fokI, one cutting DNA, the other cutting a third oligo" width="300" height="300" /></td> |
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| - | <td>[[Image:freiburg09_fokI_3oligos.JPG|none|thumb|Schema 3: 2Oligos, both with active fokI, one cutting DNA, the other cutting a third oligo|400x400px]]</td> | + | <td><img src="https://static.igem.org/mediawiki/2009/6/6e/Freiburg09_fokI_1inactive.JPG" name="Schema 4: 2Oligos, one with active fokI cutting DNA, the other inactive, just binding its own oligo" width="300" height="300" /></td> |
| - | | + | |
| - | <td>[[Image:freiburg09_fokI_1inactive.JPG|none|thumb|Schema 4: 2Oligos, one with active fokI cutting DNA, the other inactive, just binding its own oligo|400x400px]]</td>
| + | |
| | </tr> | | </tr> |
| | | | |
| | <tr> | | <tr> |
| - | <td>[[Image:freiburg09_fokI_1oligo.JPG|none|thumb|Schema 5: 1Oligo with active FokI cutting DNA, the other inactive, just binding its own oligo|400x400px]]</td> | + | <td><img src="https://static.igem.org/mediawiki/2009/8/8a/Freiburg09_fokI_1oligo.JPG" name="Schema 5: 1Oligo with active FokI cutting DNA, the other inactive, just binding its own oligo" width="300" height="300" /></td> |
| - | <td>[[Image:freiburg09_fokI_pna.JPG|none|thumb|Schema 6: 2Oligo, with active fokI cutting DNA, attached on both FokI is a specific PNA. Oligos must be designed to bind this sequence. It is to be discussed whether there is a third oligo required for 3d stabilisation of dna |400x400px]]</td> | + | |
| | + | <td><img src="https://static.igem.org/mediawiki/2009/4/4a/Freiburg09_fokI_pna.JPG" name="Schema 6: 2Oligo, with active fokI cutting DNA, attached on both FokI is a specific PNA. Oligos must be designed to bind this sequence. It is to be discussed whether there is a third oligo required for 3d stabilisation of dna" width="300" height="300" /></td> |
| | </tr> | | </tr> |
| | </table> | | </table> |
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