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Team:Waterloo/Project
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== The Experiments == | == The Experiments == | ||
===Cassette Exchange=== | ===Cassette Exchange=== | ||
| - | ====BBa Donor Plasmid==== | + | =====BBa Donor Plasmid===== |
| - | ====Landing Pad Strain==== | + | =====Landing Pad Strain===== |
| - | ====Integrase expression plasmid==== | + | =====Integrase expression plasmid===== |
===Non-crossreactive <i>att</i> sites=== | ===Non-crossreactive <i>att</i> sites=== | ||
| - | ====Integrase Mechanism==== | + | =====Integrase Mechanism===== |
| - | ====Design of new <i>att</i> sites==== | + | =====Design of new <i>att</i> sites===== |
| - | ====Design of experiment to characterize new <i>att</i> sites==== | + | =====Design of experiment to characterize new <i>att</i> sites===== |
=== Part 3 === | === Part 3 === | ||
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== Results == | == Results == | ||
Revision as of 05:02, 11 October 2009
| Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Notebook |
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Contents |
Chromobricks: A Platform for Chromosome Engineering with BioBricks
The aim of our project is to develop a fully-featured platform for chromosome engineering, allowing the in vivo assembly of a synthetic chromosome from interchangeable parts, followed by selective degradation of the native chromosome. We have designed a proof-of-concept for chromosome-building that will use the site-specific integrase of phage ΦC31 to integrate a BioBrick into a defined locus of the E. coli genome. Six pairs of integrase-targeted att sites have been designed to be non-cross-reactive in order to support repeatable cassette-exchange reactions for chromosome building. We have also written software to model the integrase-mediated rearrangement of DNA molecules containing att sites, to aid the design of more elaborate chromosome-building systems. To selectively degrade the native chromosome we designed a nuclease-based, inducible genome-degradation system. In its simplest form, our system can be used to integrate biological devices into a chromosome in situations requiring stable copy number and selection-free maintenance.
