User contributions
From 2009.igem.org
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- 07:40, 20 October 2009 (diff | hist) N Team:Todai-Tokyo/Protocols/PCR (New page: {{:Team:Todai-Tokyo/Template}} == PCR Protocol == Our PCR protocol is a combination of Taq used and the PCR program.<BR> Please refer to the following pages for details on each. * [[Te...)
- 07:32, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols/Restriction Enzyme Digest
- 07:31, 20 October 2009 (diff | hist) N Team:Todai-Tokyo/Protocols/Restriction Enzyme Digest (New page: {{:Team:Todai-Tokyo/Template}} == Restriction Enzyme Digest Protocol == 1. Mix the following in a eppendorf tube: * Xµl plasmid DNA (to a final concentration of 300ng/30ul) * 3...)
- 06:56, 20 October 2009 (diff | hist) N Team:Todai-Tokyo/Protocols/Ligation (New page: {{:Team:Todai-Tokyo/Template}} == Ligation Protocol == # Mix 2µl of insert DNA with 3µl of vector DNA # Add 5µl Takara Ligation High # Incubate at 17ºC for 30 min.)
- 06:46, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols/Transformation (→Transformation Protocol)
- 06:45, 20 October 2009 (diff | hist) N Team:Todai-Tokyo/Protocols/Transformation (New page: {{:Team:Todai-Tokyo/Template}} == Transformation Protocol == '''From iGEM Plates''' <BR> # Add 15µl TE (Tris-EDTA, pH 8.0) to well containing part and pipette up and down to resuspend...)
- 06:34, 20 October 2009 (diff | hist) N Team:Todai-Tokyo/Protocols/Miniprep (New page: {{:Team:Todai-Tokyo/Template}} == Miniprep Protocol == '''Preparation''' (previous night)<BR> # Pick Single colonies from a transformation plate and culture in 4ml Luria Broth # Cultur...)
- 06:15, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols
- 05:22, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols/Notebook Sample
- 04:05, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols/Notebook Sample
- 03:26, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols/Notebook Sample
- 03:16, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols/Notebook Sample
- 03:00, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols/Notebook Sample
- 02:47, 20 October 2009 (diff | hist) N /Protocols/Miniprep (New page: test) (top)
- 02:44, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols/Notebook Sample
- 02:43, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols/Notebook Sample
- 02:35, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols/Notebook Sample
- 02:27, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols/Notebook Sample
- 02:26, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols/Notebook Sample
- 02:15, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols/Notebook Sample
- 02:12, 20 October 2009 (diff | hist) N Team:Todai-Tokyo/Protocols/Notebook Sample (New page: {{:Team:Todai-Tokyo/Template}} {| width="100%" cellpadding="0px" cellspacing="5px" style="font-family:'Century Gothic', sans-serif;" |- style="background:#BC8F8FF; height:100px;" valign="...)
- 02:12, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Notebook
- 02:10, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols
- 02:09, 20 October 2009 (diff | hist) /Notebook Sample (top)
- 02:09, 20 October 2009 (diff | hist) N /Notebook Sample (New page: {| width="100%" cellpadding="0px" cellspacing="5px" style="font-family:'Century Gothic', sans-serif;" |- style="background:#BC8F8FF; height:100px;" valign="top" |style="width:15%;"| <html...)
- 02:06, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Notebook
- 02:03, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Notebook/isoleucine (→Plan)
- 02:02, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Notebook
- 02:01, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Notebook
- 15:14, 19 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols
- 15:04, 19 October 2009 (diff | hist) N Team:Todai-Tokyo/Protocols (New page: Here is a list of common protocols we used for our projects.<BR> Individual protocols are linked from the Notebook section in order to minimize the amount of text in the Notebook. [/Mini...)
- 14:59, 19 October 2009 (diff | hist) Team:Todai-Tokyo/Template
- 14:58, 19 October 2009 (diff | hist) Team:Todai-Tokyo/Template
- 07:39, 12 June 2009 (diff | hist) Workshops/Asia (→Accommodations) (top)
- 03:48, 8 June 2009 (diff | hist) Team:Todai-Tokyo/Notebook/isoleucine (→6/7)
- 03:39, 8 June 2009 (diff | hist) N File:090607.1.jpg (PCR of yqiT gene) (top)
- 03:37, 8 June 2009 (diff | hist) Team:Todai-Tokyo/Notebook/isoleucine (→6/7)
- 03:31, 8 June 2009 (diff | hist) Team:Todai-Tokyo
- 03:30, 8 June 2009 (diff | hist) Team:Todai-Tokyo/Notebook/isoleucine (→6/7)
- 03:28, 8 June 2009 (diff | hist) N File:090607.2.jpg (Digestion of PCR product and the GFP generator) (top)
- 03:22, 8 June 2009 (diff | hist) Team:Todai-Tokyo/Notebook/isoleucine
- 03:14, 8 June 2009 (diff | hist) Team:Todai-Tokyo/Notebook/isoleucine
- 03:01, 8 June 2009 (diff | hist) Team:Todai-Tokyo/Notebook
- 02:58, 8 June 2009 (diff | hist) Workshops/Asia (→Accommodations)
- 10:10, 7 June 2009 (diff | hist) Team:Todai-Tokyo/Notebook/isoleucine (→Creating a biobrick part out of yqiT)
- 10:09, 7 June 2009 (diff | hist) Team:Todai-Tokyo/Notebook/isoleucine (→Creating a biobrick part out of yqiT)
- 09:46, 7 June 2009 (diff | hist) N Team:Todai-Tokyo/Notebook/isoleucine (New page: ==6/5== Resuspended DNA in the following wells with 10ul water: Plate 1 1D<BR> [http://partsregistry.org/wiki/index.php/Part:BBa_R0080 AraC regulated promoter] Plate 1 12E<BR> [http://...)
- 09:17, 7 June 2009 (diff | hist) Team:Todai-Tokyo/Notebook
- 09:16, 7 June 2009 (diff | hist) Team:Todai-Tokyo/Notebook
- 06:07, 5 June 2009 (diff | hist) Workshops/Asia
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