Team:Todai-Tokyo/Protocols/Infusion

From 2009.igem.org

Revision as of 10:31, 20 October 2009 by Daichi (Talk | contribs)
UT.png
Home The Team The Project Parts Submitted to the Registry Modeling Notebook Protocols Ethics




Infusion Description

Infusion is a method to combine DNA fragments using a combination of PCR and homologous recombination. It can be used as an alternative to ligation with the advantage that it does not require restriction enzyme digests, although specific primers are required for the reaction.

[http://bioinfo.clontech.com/infusion/ Clonetech page on In-Fusion]

Infusion Protocol

  1. Mix the purified PCR insert(50-200ng) and linearized vector(100-400ng)
  2. Add 1