Team:IBB Pune/project/Results
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We carried out the following transformations successfully:Native Biobricks
1) pLL promoter - BBa_I746365 2) GFP coding sequence - BBa_E0040 3) RFP coding sequence 4) GFP Translational unit 5) RFP Translational unit 6) Enhanced CFP 7) Fast GFP 8) Constitutive promoter -BBa_J23102 9) Promoter + RBS 10) T7 promoter 11) pLuxR 12) Double terminator 13) RBS 14) Constitutive LuxI 15) LuxR Translational unit
OUR PARTS
1) YcdB 2) TorA 3) Lac Operator
Assembly
1) Const. Promoter-LacO 2) PluxR- LuxI 3) {Promoter + RBS}-GFP 4) {Promoter + RBS}-TorA 5) {Promoter + RBS}-Ycdb 6) luxR-PluxR-{RBS-LuxI} 7) Promoter + RBS 8) YcdB-GFP 9) TorA-GFP 10) {Promoter + RBS}-LuxR 11) {Promoter + RBS}-YcdB-GFP 12) {Promoter + RBS}-TorA-GFP 13) Const. Promoter-LuxR 14)
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