Team:Lethbridge/Parts

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BBa_K249002: Lumazine Synthase - Lumazine Synthase is an enzyme which creates Lumazine, a product which aggregates forming a hollow spheroid which can act as a mirocompartment, or artificial organelle. The Lumazine forms negatively charged pores, which can be used to introduce proteins. The proteins which are being introduced into the microcompartment must be equipped with an Arginine Tag. This is a protein coding part. Submitted.

BBa_K249004: N-terminal Arginine Fusion vector - pSB1A3 with an N-terminal Arginine tag. This particular plasmid utilizes the BioFusion (aka Silver Lab) Standard for BioBrick assembly at the suffix, and the regular BioBrick standard at the prefix. Adds 10 Arginine residues to the N-terminus of a Protein (includes Start Codon AUG). This part is meant to work in conjunction with the Lumazine synthase microcompartment. Protein with the N-terminal Arginine fusion will be localized into the negative core of the Lumazine microcompartments. Submitted.

BBa_K249005: C-terminal Arginine fusion vector - pSB1A3 with a C-terminal Arginine tag. This particular plasmid utilizes the BioFusion (aka Silver Lab) Standard for BioBrick assembly at the prefix and the regular Biobrick standard at the suffix. Adds 10 Arginine residues to the C-terminus of a Protein (Includes Stop Codon) this part is meant to work in conjunction with the Lumazine synthase microcompartment. Protein with the N-terminal Arginine fusion will be localized into the negative core of the Lumazine microcompartments. Submitted.

Individual Parts

BBa_K249006: Fusion EYFP with C-terminal Arginine Tag - This is a composite part. This Yellow Fluorescent Protein has a C-terminal fusion suffix (BioFusion AKA Silver Lab standard) and is ready to be fused to the C-terminal Arginine tag. This part has a ribosomal binding site. Submitted.

BBa_K249007: Fusion CFP with C-terminal Arginine Tag - This is a composite part. This cyan Fluorescent protein has a c-terminal fusion suffix (BioFusion AKA Silver Lab standard) and is ready to be fused to the C-terminal Arginine tag. This part has the pTet promoter and a ribosomal binding site. In progress

BBa_K249008: Fusion EYFP with N-terminal Arginine Tag - This is a composite part. This Yellow Fluorescent Protein has an N-terminal fusion prefix (BioFusion AKA Silver Lab standard) and is ready to be fused to the N-terminal Arginine tag. This part has a double terminator. Submitted.

BBa_K249009: Fusion ECFP with N-terminal Arginine Tag - This is a composite part. This Cyan fluorescent protein has an N-terminal fusion prefix (BioFusion AKA Silver Lab standard) and is ready to be fused to the N-terminal Arginine tag. This part has a double terminator. In progress

BBa_K249016: mms6 - This is a protein coding region. The mms6 enzyme from Magnetospirillum magneticum has been shown to produce uniform Magnetite nanoparticles when in the presence of an Iron Chloride based solution. Submitted.

BBa_K249026 : Theophylline Riboswitch - This is an improvement on the Theophylline Riboswitch submitted by the University of Lethbridge 2007 iGEM team. The theophylline Riboswitch binds to Theophylline in a specific manner to induce transcription of an mRNA. Submitted.

BBa_K249029: rpsA TIR - This part is a key regulatory element for use in the cell when under stressful conditions. This Piece of RNA allows the mRNA transcript to bypass some of the Initiation factors generally required for Ribosome recruitment. Cancelled.

Constructs/Devices

BBa_K249000: Tet Repressible YFP - This is the Yellow Fluorescent Protein from the Registry (RET) which has been modified with a Tetracycline promoter in order to be (PRET). This is a composite part. Submitted.

BBa_K249001: Arabinose Induced Inverter - The Tetracycline Inverted has been equipped with the pBAD promoter to allow for Arabinose induced Inversion of which part has been tagged on afterwards. This is a composite part. Submitted.

BBa_K249003: Lumazine Synthase - This is the Composite Part which creates the enzyme Lumazine Synthase. Lumazine Synthase generates the product Lumazine which aggregates together to form a microcompartment, or synthetic organelle. The microcompartment has large negatively charged pores through which proteins containing positively charged Arginine Tags may be targeted. This is a composite part. In progress

BBa_K249010: C-terminal Yellow Fluorescent Protein with tetracycline promoter - This is a composite part. This Yellow fluorescent Protein has been fused with a c-terminal Arginine tag (10 Additional Arginine residues at the c-terminus) and has been fixed with a tetracycline promoter, mRBS, and double terminator. In progress

BBa_K249011: C-terminal Cyan Fluorescent Protein with tetracycline promoter - This is a composite part. This Cyan Fluorescent Protein has been fused with a c-terminal Arginine tag (10 additional arginine residues at the c-terminus) and has been fixed with a tetracycline promoter, mRBS and double terminator. In progress

BBa_K249012: N-terminal Yellow fluorescent protein with tetracycline promoter - This is a composite part. This Yellow fluorescent Protein has been fused with an N-terminal Arginine tag (10 Additional Arginine residues at the N-terminus) and has been fixed with a tetracycline promoter, mRBS, and double terminator. In progress

BBa_K249013: N-terminal Cyan fluorescent protein with tetracycline promoter - This is a composite part. This Cyan fluorescent Protein has been fused with an N-terminal Arginine tag (10 Additional Arginine residues at the N-terminus) and has been fixed with a tetracycline promoter, mRBS, and double terminator. In progress

BBa_K249014: C-terminal Yellow fluorescent protein with tetracycline inverter - Arabinose repressible Tetracycline inversion of YFP with C-terminal Fusion Arganine Tag for targeting into Lumazine Microcompartment. Has a medium RBS and a double terminator. Submitted.

BBa_K249015: C-terminal Cyan fluorescent protein with tetracycline inverter - Arabinose repressible Tetracycline inversion of CFP with C-terminal Fusion Arganine Tag for targeting into Lumazine Microcompartment. Has a medium RBS and a double terminator. In progress

BBa_K249017: N-terminal Yellow fluorescent protein with tetracycline inverter - Arabinose repressible Tetracycline inversion of YFP with N-terminal Fusion Arganine Tag for targeting into Lumazine Microcompartment. Has a medium RBS and a double terminator. Submitted.

BBa_K249018: N-terminal Cyan fluorescent protein with tetracycline inverter - Arabinose repressible Tetracycline inversion of CFP with N-terminal Fusion Arganine Tag for targeting into Lumazine Microcompartment. Has a medium RBS and a double terminator. In progress

BBa_K249019: Magnetite nanoparticle Device - This is a composite part. The mms6 enzyme from Magnetospirillum magneticum has been shown to produce uniform Magnetite nanoparticles when in the presence of an Iron Chloride based solution. The coding region has been equipped with an IPTG inducible promoter, strong RBS and double terminator. Thank you to the University of Alberta Teamfor collaborating with us on this part. Submitted.

BBa_K249020: Targeted C-terminal CFP and Lumazine Synthase - This is a composite part. This is device has been designed to show the localization of the Cyan fluorescent Protein with C-terminal Arginine tag into the Lumazine Microcompartment. In progress

BBa_K249021: Targeted C-terminal YFP and Lumazine Synthase - This is a composite part. This is device has been designed to show the localization of the yellow fluorescent Protein with C-terminal Arginine tag into the Lumazine Microcompartment. In progress

BBa_K249022: Targeted N-terminal CFP and Lumazine Synthase - This is a composite part. This is device has been designed to show the localization of the Cyan fluorescent Protein with N-terminal Arginine tag into the Lumazine Microcompartment. In progress

BBa_K249023: Targeted N-terminal YFP and Lumazine Synthase - This is a composite part. This device has been designed to show the localization of the yellow fluorescent Protein with N-terminal Arginine tag into the Lumazine Microcompartment. In progress

BBa_K249024: Targeted C-terminal CFP and targeted C-terminal YFP and Lumazine Synthase - This is a composite part. This is device has been designed to show the co-localization of the Cyan fluorescent Protein and Yellow fluorescent protein both equipped with C-terminal Arginine tag into the Lumazine Microcompartment. In progress

BBa_K249025: Targeted N-terminal CFP and targeted N-terminal YFP and Lumazine Synthase - This is a composite part. This is device has been designed to show the co-localization of the Cyan fluoresent Protein and Yellow fluorescent protein both equipped with N-terminal Arginine tag into the Lumazine Microcompartment. In progress

BBa_K249027: Theophylline Induced GFP - GFP is controlled through translation by the Theophylline Riboswitch. When Theophylline is present, the Riboswitch undergoes a conformational change to reveal the RBS, allowing production of GFP. In progress

BBa_K249028: Theophylline Induced Chemotaxis - Chemotaxis by cheZ is controlled through translation by the Theophylline Riboswitch. When Theophylline is present, the Riboswitch undergoes a conformational change to reveal the RBS, allowing the production of cheZ, a key regulatory element in the motility pathway. Submitted.

BBa_K249030: rpsA TIR controlled GFP - Green Fluorescent Protein expression is controlled by the rpsA TIR which allows for the protein to be expressed under stressful cellular conditions. Cancelled.

Intermediate Parts

BBa_S04259: Lumazine-dT : This is the Lumazine coding region with a double terminator. Composed of K249002:B0015. Submitted.

BBa_S04260: sRBS-Lumazine-dT : This is the Lumazine coding region with w strong RBS and double terminator. Composed of B0030:K249002:B0015. In progress

BBa_S04261: C-terminal-dT : This is the C-terminal Arginine fusion vector with a double terminator added to it. Composed of K249005:B0015. Submitted.

BBa_S04262: mRBS-N-terminal : This is the N-terminal Arginine fusion vector with a medium RBS added to it. Composed of B0032:K249004. Submitted.

BBa_S04263: GFP-dT: This is an improvement to a part already in the registry. We felt it necessary to remake this part since we had some trouble with it in 2008, and there was no evidence that anyone had ever had success with it on the registry. Composed of E0040:B0015. Submitted.

BBa_S04264: fusion EYFP-dT: This is the yellow fluorescent protein with the C-terminal Arginine tag, a double terminator has been added on to the end. Composed of K249006:K249005:B0015. Submitted.

BBa_S04265: fusion ECFP-dT: This is the cyan fluorescent protein with the C-terminal Arginine tag, a double terminator has been added on to the end. Composed of K249007:K249005:B0015. In progress

BBa_S04266: mRBS-N-terminal EYFP: This is the yellow fluorescent protein with the N-terminal Arginine tag, a medium RBS has been added. Composed of B0032:K249004:K249008. Submitted.

BBa_S04267: mRBS-N-terminal ECFP: This is the cyan fluorescent protein with the N-terminal Arginine tag, a medium RBS has been added. Composed of B0032:K249004:K249007. In progress

BBa_S04268: mms6-dT: This is the mms6 gene from magnetospirillum magneticum which produces magnetite nanoparticles. It has been equipped with a double terminator. Composed of K249016:B0015. Submitted.

BBa_S04269: sRBS-mms6-dT: This is the mms6 gene from magnetospirillum magneticum which produces magnetite nanoparticles. It has been equipped with a double terminator and a strong RBS. Composed of B0030:K249016:B0015. In progress

BBa_S04270: Riboswitch-GFP-dT: This is the theophylline Riboswitch (which acts as an alternative RBS) which has been equipped with the fluorescent reported GFP and a double terminator. Composed of K249026:E0040:B0015. Submitted.

BBa_S04271: cheZ-dT: This is a composite part in the motility pathway. Composed from the 2008 Lethbridge teams cheZ submission and a double terminator. Composed of K149000:B0015. Submitted.

BBa_S04272: Riboswitch-cheZ-dT: This is the theophylline riboswitch (which acts as an alternative RBS) which has been equipped with the cheZ motility gene and a double terminator. Composed of K249026:K149000:B0015. Submitted.

BBa_S04273: rpsA TIR-GFP-dT: This is the rspA TIR equipped with the fluorescent reporter gene GFP and a double terminator. Composed of K249029:E0030:B0015. Cancelled.