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Notebook Flow Cytometry
9-24-2009
- We performed standard measurements in HeLa, U2OS and MCF7 with our three standard promoters: CMV, JeT and JeT-CMV. These measurements will be performed in 3 independent measurements. The initial result of U2OS is shown below, which includes the following samples (see table 1).
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| Sample
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| negative control (transfection with a non-fluorescent plasmid p6)
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| cotransfection with JeT plasmid and p6
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| cotransfection with JeT_CMV plasmid and p6
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| cotransfection with CMV plasmid and p6
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| cotransfection with mCherry reference plasmid and p6
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| cotransfection with CMV plasmid and mCherry reference plasmid
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| cotransfection with JeT plasmid and mCherry reference plasmid
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| cotransfection with JeT_CMV plasmid and mCherry reference plasmid
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 Figure ?: Flow cytometry results of the U2-OS standard plate The . |
- We measured the constitutive promoters in the HeLa cell line. Unfortunately, the number of the viable cells are not enough for a reliable measurement.
9-25-2009
- We measured the MCF-7 Standard plate, which includes the same samples as above (see table 1)
 Figure ?: Flow cytometry results of the MCF-7 standard plate The .
9-28-2009
- We measured the MCF-7 and the U2-OS Standard plate again (same samples).
 Figure ?: Flow cytometry results of the U2-OS standard plate The . |
 Figure ?: Flow cytometry results of the MCF-7 standard plate The . |
9-29-2009
- We measured the MCF-7 and the U2-OS Standard plate the third time (same samples).
 Figure ?: Flow cytometry results of the U2-OS standard plate The . |
 Figure ?: Flow cytometry results of the MCF-7 standard plate The . |
9-30-2009
- We measured the constitutive promoters in the HeLa cell line again.
10-02-2009
- We measured the U2-OS NFkB plate 10 hours after induction.
Figure ?: Flow cytometry results of the U2-OS standard plate The .
10-03-2009
- We measured the HIF promoter in MCF-7. Unfortunately, the cell number was too low.
10-05-2009
- We measured the HeLa Standard plate in three different experiments????, which includes the same samples as above (see table 1)
- We measured the constitutive promoters in the HeLa cell line again. Unfortunately, the number of the viable cells are not enough for a reliable measurement.
- We measured the AhR ??? promoter in HeLa. Unfortunately, the number of the viable cells are not enough for a reliable measurement.
10-07-2009
- We measured the U2-OS NFkB plate 10 hours after induction again.
10-08-2009
- We measured the Estrogen promoter in MCF-7. Unfortunately, the number of the viable cells are not enough for a reliable measurement.
10-09-2009
- We measured the HeLa Standard plate (fourth time).
- We measured the SREBP ??? in HeLa.
- We measured the constitutive promoters in HeLa again.
10-12-2009
- We measured the HeLa Standard plate (fifth time).
- We measured the promosing NKkB promoter NIIL10 ??? in different medium in U2-OS. Here, we want to see, if there is an induction of a nonspecific drug (Thiazolidin??) and how the different medium affect the promoter activity. No significant change.
10-13-2009
- We measured the p53 ?? promoter in MCF-7. Unfortunately, the flow cytometry machine broke up the measurement and so we didn't have all the important data.
10-14-2009
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