Team:Imperial College London/Temporal Control
From 2009.igem.org
![](https://static.igem.org/mediawiki/2009/b/b8/II09_ImperialLogo.png)
Temporal Control
In our project there are 3 forms of temporal control that have been implemented.
- Chemical induction: Triggers the production of the polypeptide of interest using IPTG. Effectively 'kicks off' the system once the cell density is high enough.
- Autoinduction: Represses encapsulation when glucose levels are high, and kick starts it once glucose is used up. This allows a sufficient amount of protein production to have taken place before the cell focuses its resources on encapsulation.
- Thermoinduction: Triggers genome deletion when the temperature is increased. Thermoinduction was necessary, as chemical induction may be blocked by the presence of the capsule (that inhibits diffusion).
Project Tour
![](https://static.igem.org/mediawiki/2009/d/d9/II09_Temp_ArrowLeft.png)
![](https://static.igem.org/mediawiki/2009/c/ce/II09_Temp_ArrowRight.png)
For more details of the temporal control of the system, see the tabs below.
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![](http://i691.photobucket.com/albums/vv271/dk806/II09_Homepageimage3.png)
![](http://i691.photobucket.com/albums/vv271/dk806/II09_chemicalinduction.png)
![](http://i691.photobucket.com/albums/vv271/dk806/II09_Drylabmainimage1.png)
![](http://i691.photobucket.com/albums/vv271/dk806/II09_Thermoinduction1.png)
![](http://i691.photobucket.com/albums/vv271/dk806/II09_Wetlabmainimage9.png)
![](http://i691.photobucket.com/albums/vv271/dk806/II09_Drylabmainimage6.png)
Temporal Control Contents
![](http://i691.photobucket.com/albums/vv271/dk806/II09_Homepageimage3.png)
![](http://i691.photobucket.com/albums/vv271/dk806/II09_chemicalinduction.png)
![](http://i691.photobucket.com/albums/vv271/dk806/II09_Drylabmainimage1.png)
![](http://i691.photobucket.com/albums/vv271/dk806/II09_Thermoinduction1.png)
![](http://i691.photobucket.com/albums/vv271/dk806/II09_Wetlabmainimage9.png)
![](http://i691.photobucket.com/albums/vv271/dk806/II09_Drylabmainimage6.png)