HKU-HKBU/29 July 2009

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Contents

1 Co-transformation Transform two plasmids (A+, C+) to MG3.① Plac-CheZ-His-TT C+② Lac Iq A+
2 14:10 Subculture to warm room (37℃), shaking for 1.5h.
3 15:40 take the subculture bacteria out from the warm room.Freeze the bacteria in 0℃ ice water for
4 15min.
5 Centrifuge in 4000rmp at 0℃ for 2min.Decent supernatant

Co-transformation Transform two plasmids (A+, C+) to MG3.① Plac-CheZ-His-TT C+② Lac Iq A+

14:10 Subculture to warm room (37℃), shaking for 1.5h.

15:40 take the subculture bacteria out from the warm room.Freeze the bacteria in 0℃ ice water for

15min.

Centrifuge in 4000rmp at 0℃ for 2min.Decent supernatant

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