Team:Todai-Tokyo/Protocols/Restriction Enzyme Digest

From 2009.igem.org

Revision as of 07:31, 20 October 2009 by Craig (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
UT.png
Home The Team The Project Parts Submitted to the Registry Modeling Notebook Protocols Ethics




Restriction Enzyme Digest Protocol

1. Mix the following in a eppendorf tube:

  • Xµl　plasmid DNA （to a final concentration of 300ng/30ul）
  • 3µl　10 H　buffer
  • 1.5µl　Restriction Enzyme 1
  • (1.5µl　Restriction Enzyme 2, if applicable)
  • MilliQ　up　to　30ul

2. Incubate at 37ºC for 1h or more

Retrieved from "http://2009.igem.org/Team:Todai-Tokyo/Protocols/Restriction_Enzyme_Digest"