Uppsala-Sweden/20 September 2009

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Evaluation of yesterdays PCR

20090920 colony pcr eval.jpg

All the colonies corresponding to the gel above were inoculated at 12:00.


Evaluation of PCR for pPetE and ADH2Z

20090920 pcr eval.png


The pPetE PCR product were digested with EcoRI + PstI and ligated into psb1A3. After that the ligated plasmid were transformed into Top10 competent cells through heat-shock for 55 sec in 42 deg C and incubated over night.

Picking colonies

Colonies were picked from the transformations made yesterday.





Bioneer Biolegio Clontech Uppsala Genome Center

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