Team:Imperial College London/Wetlab/Results/Cheminduction

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Experiment:

Different concentrations of IPTG were added to a 96-well plate [link to assay] of e-coli Top 10 cells containing LacI-RFP genetic constructs. Here we measured the optical density of the cells over-time, for different concentrations of IPTG to monitor the effects in the culture. The IPTG growth curve results are plotted below:
II09 IPTG growth1.jpg
Figure 1: Effects of [IPTG] on cell growth
: Note that the high initial OD value results due to cultures having grown for 1 hour prior the start of the measuring phase.

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