Procedures
Polymerase Chain Reaction (PCR)
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Transformation
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Biobrick Assembly
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Gel Electrophoresis
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Recipes
Lysogeny Broth (LB) Media
- 10g Tryptone
- 5g Yeast Extract
- 10g NaCl
- 15g Agar
- up to 1 Liter H20
ATCC medium: Van Niel's yeast agar
- 1.0 g K2HPO4
- 0.5 g MgSO4
- 10.0 g yeast extract
- 20.0 g agar
- 1.0 L tap water
- Adjust pH to 7.0-7.2
Super Optimal Broth (SOB)
- 2% w/v bacto-tryptone (20g)
- 0.5% w/v bacto-yeast extract (5g)
- 8.56mM NaCl (0.5g) or 10mM NaCl (0.584g)
- 2.5mM KCl (0.186g)
- ddH2O to 1L
Super Optimal Broth with Catabolite repression (SOC)
- In addition to all of the SOB components,
- 10mM MgCl2 (0.952g)
- 20mM glucose (3.603g)
Gel (for Electrophoresis)
- 50 mL 1X TAE buffer
- 0.8 g agarose
- 2.5 microliters EtBr
Tris EDTA (TE) buffer
- 750 mL d-H20
- 242 g Tris base
- 57.1 mL glacial acetic acid
- 100 mL 0.5M EDTA (93.05 g EDTA in 500 mL d-H20, pH ~8.0)
- fill to 1 L
- adjust pH to 8.5