August/12 October 2009

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1. min prep

sample        conc.
 C2               14 ng/uL
 C3               14
 C4               13
 68              182
 68              118
 69              161
 69              119
 74              250
 74              243
 78              105


2.digation

K204072

VectorInsert
2-8E63110
EcoRI1EcoRI1
XbaI1SpeI1
No.25No.25
dH2O32dH2O28
total50uLtotal50uL


K204075

VectorInsert
1-1K101-8K2
SpeI1XbaI1
PstI1PstI1
No.2 2No.22
dH2O28dH2O36
total50uLtotal50uL


K204085

VectorInsert
4031-15L10
EcoRI1EcoRI1
XbaI1SpeI1
No.25No.25
dH2O35dH2O28
total50uLtotal50uL


K204086

VectorInsert
2-3I3693
SpeI1XbaI1
PstI1PstI1
No.2 2No.22
dH2O28dH2O36
total50uLtotal50uL


K204087

VectorInsert
622476
SpeI1XbaI1
PstI1PstI1
No.2 2No.22
dH2O36dH2O32
total50uLtotal50uL


K204088

VectorInsert
X32693
SpeI1XbaI1
PstI1PstI1
No.2 2No.22
dH2O36dH2O35
total50uLtotal50uL


K204089

VectorInsert
1-24E2694
SpeI1XbaI1
PstI1PstI1
No.2 2No.22
dH2O34dH2O34
total50uLtotal50uL


K204090

VectorInsert
1-23L5chiba5
EcoRI1EcoRI1
XbaI1SpeI1
No.25No.25
dH2O33dH2O3
total50uLtotal50uL


37degree , 3hr
gel cut & purification
ligation O/N

3. min prep

sample      conc,
 58              127 ng/uL
 58              229
 62              79
 76              202
 77              175 
 77              184


4.transformation

sample
79 , 80 , 81 , 82 , 83 , 84 , 68 , 71 , 67 , 73 


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