IBB Pune/19 June 2009

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I) Gel elution. (Kit used, Bangalore Genei)

A280 of eluted DNA

  • 0.0138
  • 0.0123
  • 0.0134
  • 0.0365

~500 ng DNA !

Too good to be true, running a gel to confirm.


II) pBR322 isolated successfully.

III) Ran a PCR with Vf and Vr.

Gel positive.

DNA is present, QED.

Try P1 and P2, lets see if a gradient PCR will solve our problem...