Team:KU Seoul/Parts

Part

Mercury sensor - Mercury inducing promoter : Part:[http://partsregistry.org/Part:BBa_I728456 BBa_I728456] + amd gene [http://partsregistry.org/Part:BBa_K271000 BBa_K271000]

Cadmium is an exceedingly toxic heavy metal with no known biological function. At low cadmium concentrations,

cells are able to adapt and resume growth after a period of stasis. Promoter yodA is only transiently activated

during cadmium-induced growth inhibition. Aryl acylamidase (EC 3.5.1.13; AAA) is an enzyme that acts on the

amide bond between aryl- and acyl groups. The most typical reaction is the hydrolysis of an anilide, producing

a carboxylate and aniline, which is also reversible. Acetaminophen(Tyrenol) is an aryl acyl compound having

amide bond is hydrolyzed by the enzyme and produce acetate and p-aminophenol. Because p-Aminophenol have red-

purple color, aryl acylamidase gene will apply to reporter gene.

Heavy-metal detector [http://partsregistry.org/Part:BBa_K271001 BBa_K271001]

The promoter/operator region of the zntA gene of Escherichia coli and shown that Zn(II) is the primary inducer

of expression of this Zn(II)/Cd(II) export gene. The promoter PzntA shows sequence similarities to the

promoters of mercury resistance(mer) operons, including a long spacer region containing an inverted repeat

sequence.