Team:IBB Pune/project/Results
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Characterization of And Gates:
Even as the wiki freezes we are characterizing Const.Prom-LacO-mRFP for its expression.
We carried out the following transformations successfully:
Native Biobricks
- pLL promoter - BBa_I746365
- GFP coding sequence - BBa_E0040
- RFP coding sequence
- GFP Translational unit
- RFP Translational unit
- Enhanced CFP
- Fast GFP
- Constitutive promoter -BBa_J23102
- Promoter + RBS
- T7 promoter
- pLuxR
- Double terminator
- RBS
- Constitutive LuxI
- LuxR Translational unit
OUR PARTS
- YcdB
- TorA
- Lac Operator
Assembly
- Const. Promoter-LacO
- PluxR- LuxI
- {Promoter + RBS}-GFP
- {Promoter + RBS}-TorA
- {Promoter + RBS}-Ycdb
- luxR-PluxR-{RBS-LuxI}
- Promoter + RBS
- YcdB-GFP
- TorA-GFP
- {Promoter + RBS}-LuxR
- {Promoter + RBS}-YcdB-GFP
- {Promoter + RBS}-TorA-GFP
- Const. Promoter-LuxR
Failed Transformations
Backbones
- pSB1AK3 with CcdB gene
- pSB1T3 with CcdB gene
- pSB1C3 with CcdB gene
- pSB1K3 with CcdB gene
- pSB1A3 with CcdB gene
- pSB1AC3 with CcdB gene
- pSB1AK3 with mRFP
- pSB1T3 with mRFP
- pSB1C3 with mRFP
- pSB1K3 with mRFP
- pSB1A3 with mRFP
- pSB1AC3 with mRFP
Native Biobricks
- Lysis Cassette
- Constitutive GFP
- COnstitutive CFP
GEL PICTURES
Lane1 Lane2 Lane3 Lane4 Lane5 Lane6 Lane7 TorA YcdB YcdB LacO LacO pSB1AK3 pSB1AK3 Retrieved from "http://2009.igem.org/Team:IBB_Pune/project/Results"