Team:EPF-Lausanne/Last News

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Keep track with what we did so far

(12.07.09)

   This first week of wetlab we have done the following things

   * Transformed the plasmids with the LovTAP gene, generously sent by Dr. Sosnick's lab from Chicago universtiy, into competent E.Coli
   * Designed the cloning strategy for cloning the LovTAP gene from its original vector to a iGEM vector+add an inducible promoter (LacI) (+RBS +term.)
   * Ordered and received the primers needed for the PCR of LovTAP
   * Designed the cloning strategy for inclusion of the LovTAP BioBrick with different reporting cassettes
   * Transformed all the BioBricks that will be needed for the cloning strategies (c.f. notebook for more information about these parts) into competent E.Coli
   * Fused the two BioBricks "LacI" and "RBS"
   * Digested the LovTAP PCR products and RBS part