Team:Paris/Production overview

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Overview


Constructions


  • The creation of OMV's

To create the vesicules, we started with a first simple construction only containing the unfunctional TolR II biobrick placed donwstream a pTetR promoter. With the presence of arabinose in the medium, pBad is activated and TetR, a repressor of the pTetR pomoter in created. The addition of of tetracycline or its analog, aTc, in th emedium inhibits the repression thus allowing the creation of vesicules.


  • The delay