Team:Paris/Production overview

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Overview

Constructions

• Creation of OMVs

To create the vesicules, we started with a first simple construction only containing the unfunctional TolR II biobrick placed donwstream a pTetR promoter. With the presence of arabinose in the medium, pBad is activated and TetR, a repressor of the pTetR pomoter in created. The addition of of tetracycline or its analog, aTc, in the medium inhibits the repression thus allowing the creation of vesicules.

For further precisions on the biobricks used as well as the plasmid backbones, please visit our parts design page [[Team:Paris/Production_design] Link title]

• The Delay