Team:TUDelft/24 August 2009

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Lab Notebook

July
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August
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September
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October
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24 August 2009

Transformaltion of friday 21-08-2009 looked good. On each plate there were 80-90 kolonies

Daniel

Digestion of assemblies in order to do the second round of assembly. Gel 1% (left) for Calin´s PCR and 2% (right) for my digestions and Calin´s PCR

Gel240809.jpg

Right Gel (2%)

Well Assembly Expected Plasmid Size Status
1 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder]
2 4A 824  ?
3 5A 1040
4 2A 214  ?
5 3A 183  ?
6 1A 1076  ?
7 PCR 1
8 PCR 2
9 PCR 3
10 PCR 4
11 PCR 5
12 PCR 6