EPF-Lausanne/31 August 2009

Wet Lab

Digestion

The Klenow fragments that have been amplified by PCR (after the Klenow reaction) will be digested with SpeI. We have to check if it is indeed the TrpO (presence of the two S sites).

Results of the overweekend cultures

The idea was to find a new medium without any TRY on it, but on which the cells can still grow.

The minimal media + arginine didn't show any result (nothing grew).

The tubes induced by Try didn't change colour over the week-end. The two that were already red are still red and the others didn't change colour.

RO1 construction

RO2 construction

People in the lab

Basile