August/9 August 2009

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1.colony check

 ( sample No.)  (no. of colonies)
       9                50<
       24                0 
       29                0
       30                0
       31                8
       32                2
        
        9                1

2.digestion and ligation

digestion with restriction enzyme

K204024

VectorInsert
terminater(1-23L)6No. 14-A 12
EcoRI1EcoRI1
XbaI1SpeI1
No.22No.22
dH2O10dH2O4
total20uLtotal20uL


K204029

VectorInsert
2-18F62-1610
EcoRI1EcoRI1
XbaI1SpeI1
No.22No.22
dH2O10dH2O6
total20uLtotal20uL


K204030

VectorInsert
2-18F101-19B6
SpeI1XbaI1
PstI1PstI1
No.2 2No.22
dH2O6dH2O10
total20uLtotal20uL


37degree 3hr

Agarose Gel Electro-phoresis

Osaka090909.jpg

No. 24 , 29 , 30 , 10-D , 10-E , ladder


16 degree O/N

3.Min prep
Result of Nanodrop concentration check

 (sample No.)      (concentration)
     1-1D                 27.5
     10-D                 85.1
     10-E                 117.2


4.transformation

ligation sample No. 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40A ,40B
sample No. 24 , 29 , 30



5.digestion and ligation

digestion with restriction enzyme


K204041

VectorInsert
1-1D121-12M8
SpeI1XbaI1
PstI1PstI1
No.2 2No.22
dH2O4dH2O8
total20uLtotal20uL


K204042

VectorInsert
1-1D121-8I8
SpeI1XbaI1
PstI1PstI1
No.2 2No.22
dH2O4dH2O8
total20uLtotal20uL


37degree 3hr

Agarose Gel Electro-phoresis

Osaka090909-2.jpg

No. 41 , 42 , ladder


16 degree O/N