Team:EPF-Lausanne/Last News

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Last News




Keep track with what we did so far

(29.08.09)

This eighth week of wetlab we have done the following things:
  • Cultures to clone the biobrick in front of RO2 -> didn't work
  • Double transformation
  • Send to sequencing
  • RO1 is yet not working


(22.08.09)

This seventh week of wetlab we have done the following things:
  • Beginning of the RbphP project (PCR colony)
  • Try of 1.5 step PCR to get the complete biobrick from LovTap -> didn't work
  • Check (digestion assay) to confirm we have the LovTap-term
  • Try of medium M9 for RO2
  • Characterisation (fluorescence in fonction of induction) for RO2
  • From LovTap-term, ligation to obtain the biobrick LacI-Rbs-LovTap-term with cloning steps (not from the 1.5 step PCR)


(15.08.09)

This sixth week of wetlab we have done the following things:
  • Protocol of ligation was refined.
  • !!! Inducible LOVTAP-term and Read out 2 biobrick were created.!!! The system is completed.
  • They need to be sequenced, characterized and submitted to the registery.


(08.08.09)

This fifth week of wetlab we have done the following things:
  • Problem in ligations.


(01.08.09)

This fourth week of wetlab we have done the following things:
  • Our gene of interest (photoreceptor LOVTAP) was cloned in front of a terminator (iGEM part [http://partsregistry.org/Part:BBa_B0015 BBa_B0015]). We created our first biobrick.
  • The second biobrick was coloned as well, but we wait for the results to confirm that the plasmid contain the right insert
  • Modeling part: Preliminar simulations were lauched, and their analysis were done. Simulations will be lauched in few days.


(24.07.09)

This third week of wetlab we have done the following things:
  • Again : Several attempts to create the first biobrick (Our gene of interest (photoreceptor LOVTAP) in a iGEM plasmid containing a terminator) and the second biobrick (A lacI promoter in front of an RBS), but no results were obtained for the first biobrick neither for the second one.
  • Modeling part: Preliminar simulation was launched over the weekend.


(17.07.09)

This second week of wetlab we have done the following things:
  • Several attempts to create the first biobrick (Our gene of interest (photoreceptor LOVTAP) in a iGEM plasmid containing a terminator) and the second biobrick (A lacI promoter in front of an RBS), but no results were obtained for the first biobrick neither for the second one.
  • Modeling part: Files required to launch simulations were created and analyzed.


(12.07.09)

This first week of wetlab we have done the following things:
  • Transformed the plasmids with the LovTAP gene, generously sent by Dr. Sosnick's lab from Chicago universtiy, into competent E.Coli
  • Designed the cloning strategy for cloning the LovTAP gene from its original vector to a iGEM vector+add an inducible promoter (LacI) (+RBS +term.)
  • Ordered and received the primers needed for the PCR of LovTAP
  • Designed the cloning strategy for inclusion of the LovTAP BioBrick with different reporting cassettes
  • Transformed all the BioBricks that will be needed for the cloning strategies (c.f. notebook for more information about these parts) into competent E.Coli
  • Fused the two BioBricks "LacI" and "RBS"
  • Digested the LovTAP PCR products and RBS part

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