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Edna Miao's notebook
From 2009.igem.org
9/10/09 I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: ho46 ho176 ho180 ho236 ho252 ho269 ho270 ho320 ho335
9/9/09
I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness:
ho46
ho171
ho173
ho178
ho180
ho181
ho251
ho242
ho239
ho268-ho270
ho240
9/8/09
I froze down ho270 for long term storage.
ho253, 241, 200 got contaminated, and were subsequently tossed out.
9/4/09
I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: ho46 ho245 ho242 ho168 ho230 ho199 ho194 ho266 ho201 ho203 ho268 ho269
9/3/09
I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: ho46 ho159 ho176 ho182 ho207 ho209 ho203 ho227 ho237 ho348
I froze down these strains for long term storage: ho268-269
9/2/09
I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: ho46 ho266 ho267 ho262 ho265
9/1/09
I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: ho172 ho192 ho196 ho198 ho200 ho197 ho46 ho250 ho249 ho243 ho245 ho248 ho261 ho262 ho264 ho265
Ho260 was contaminated, and ho263 had problems, so we threw both strains and their respective data away.
8/31/09
I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness:
ho239-243, 245, 248, 250, 253
I froze down these strains for long term storage: ho266, ho267
8/28/09
I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness:
ho239-ho265
ho46
8/27/09
I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: ho174 ho175 ho168 ho212 ho194 ho211 ho46
I froze down these cells for long term storage: ho239-ho265
8/26/09
I transformed plasmids ho312 and ho313 into HO46 cells.
8/21/09
I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: ho12 ho351 ho352 ho354 ho356-ho363 ho371 ho372 ho46 ho160 ho194 ho199 ho236 ho230 ho46 from alex
8/20/09
I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: ho12 ho356-ho363 ho371 ho372
I plated ho12, ho356-363, ho371, ho372 for fruiting bodies.
8/19/09
I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: ho12 ho351 ho352 ho354
8/17/09
I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: ho12 ho337-341 ho343 ho344 ho346
I tossed these strains and their subsequent data: ho350 ho353 ho355 ho364 - 370 ho373 because they exhibit phenotype similar to P13K1/2- mutants, an error could have been made in a transformation - transformed into HO2 cells instead of HO12.
8/14/09
I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: ho46 ho230 ho12 ho337-346 ho310-316 ho321-324
I plated ho46, ho211 for the fruiting bodies assay.
8/13/09
I plated ho12, ho337 - ho346 to check their fruiting bodies
8/12/09
I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: ho12 ho310 - ho316, ho321-ho324
8/10/09
Fruiting Body Assay:
HO46:39 plaques with fruiting bodies.
HO199:3/14 plaques have small fruiting bodies, with one plaque having an abnormally large set of fruiting bodies.
HO 194:0/26 have anything unusual.
HO21:0/29 plaques have anything unusual.
HO211 needs to be redone - only 11 plaques.
HO230:0/30 plaques have anything unusual.
HO235:0/35 plaques have anything unusual.
HO236:0/29 plaques have anything unusual.
HO12:21 plaques have no fruiting bodies.
HO299:72/90 plaques have fruiting bodies.
For more assays, refer to Ethan Chan's notebook.
8/7/09
I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: HO12, HO300-HO308
