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Overall Description & Design
TNT-Sensing
Nitrite/Nitrate-Sensing
Materials and Methods
Biobricks
Problem Solving and Tips
Results
Future Work
References
Home
Biology
Overall Description and Design
TNT-Sensing
Nitrite/Nitrate-Sensing
Materials and Methods
Biobricks
Problem Solving and Tips
Results
Future Work
References
Modelling
Overall Description
Gene Regulatory Network
Real Life Modelling
Scale Up
Results
References
Underlying Philosophy
Public Perception
Legislation issues
Biosafety
Religious Perception
Informatics
Introduction
Concepts and Technologies
Globe Tutorial
iGEM WIKI Hacks
Conclusions
Notebook
Team
Team Members
Advisors
Supervisors
Gallery
Contacts
Acknowledgements
Biology - Material and Methods
Personal note
CF
Protocol Name
Download Link
Comments
Creating Composite BioBrick
Transformation of Cells
Cloning BioBrick Parts by Fusion PRC
Protocol to Store Cell Cultures
Removal of PstI Site on onr Enzyme
Sequencing Protocol
Competent Cells Preparation
Transduction of
E.Coli
Making of P1 Lysate
Quick
E.coli
transformations using CaCl2
PCR
Primers
Download Link
Comments
E.Coli
Nitrate-Nitrite Sensing Upstream Primers
Enterobacter Cloacae Pentaerythritol Tetranitrate Reductase
Lux Operon Primers
Pentaerythritol Tetranitrate Reductase Primers
TNT.R1 A Receptor With Fair Affinity
TrzI Primers
Enterobacter Cloacae Pentaerythritol Tetranitrate Reductase (onr) Gene
Photobacterium Phosphoreum
Lumazine Protein (lumP) Gene
Edinburgh University iGEM Team 2009