August/13 October 2009

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1.colony check

sample     no. of colony
  67             ++
  68             ++
  71             +
  73             +
  79             + 
  80             +
  81             +
  82             +
  83             +
  84             +


2.digation
K204072

VectorInsert
2-8E5315
EcoRI1EcoRI1
XbaI1SpeI1
No.25No.25
dH2O33dH2O33
total50uLtotal50uL

K204090

VectorInsert
1-23L5chiba 35
EcoRI1EcoRI1
XbaI1SpeI1
No.25No.25
dH2O33dH2O33
total50uLtotal50uL

37degree , 2hr
gel cut& purification
ligation

3.min prep

sample    conc.
 68           53 ng/uL
                142
                 76
                 59


4.digation

K204091

VectorInsert
655765
EcoRI1EcoRI1
XbaI1SpeI1
No.25No.25
dH2O33dH2O33
total50uLtotal50uL

K204092

VectorInsert
7736510
SpeI1XbaI1
PstI1PstI1
No.2 2No.22
dH2O35dH2O38
total50uLtotal50uL

K204093

VectorInsert
762676
SpeI1XbaI1
PstI1PstI1
No.2 2No.22
dH2O36dH2O32
total50uLtotal50uL

K204094

VectorInsert
773676
SpeI1XbaI1
PstI1PstI1
No.2 2No.22
dH2O35dH2O32
total50uLtotal50uL

K204095

VectorInsert
7621-19B6
SpeI1XbaI1
PstI1PstI1
No.2 2No.22
dH2O36dH2O32
total50uLtotal50uL

K204096

VectorInsert
7721-19B6
SpeI1XbaI1
PstI1PstI1
No.2 2No.22
dH2O36dH2O32
total50uLtotal50uL



37 degree , 2hr
gel cut & purification
ligation

5.min prep

sample         conc.
 67                 87  ng/uL
 68                 28
 71                 64
 73                 12
 79                   5
 80                   9
 81                 75
 82                 14
 83                 19
 84                 15



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