August/1 October 2009

From 2009.igem.org

Revision as of 00:25, 21 October 2009 by Unocchi (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

1.colony check

sample no.      no. of colony
    41                       +++
    42                       +++
    48                       +++
   1-1K                    +++
   1-6I                     +++
     57                       +


2.min prep

sample no.         conc.
       52                61.4 ng/uL

3.fluorence check (color parts)

 not confirm fluorence...


4.prepared LA plate

5.transformation

sample
1-14N , 1-16I


6.digation

K204058

VectorInsert
1-8E41-8I10
SpeI1XbaI1
PstI1PstI1
No.2 2No.22
dH2O12dH2O6
total20uLtotal20uL


K204059

VectorInsert
1-8E91-8K2
SpeI1XbaI1
PstI1PstI1
No.2 2No.22
dH2O7dH2O14
total20uLtotal20uL


K204060

VectorInsert
1-8E81-12M4
SpeI1XbaI1
PstI1PstI1
No.2 2No.22
dH2O8dH2O12
total20uLtotal20uL


K204061

VectorInsert
1-8E82-8E8
SpeI1XbaI1
PstI1PstI1
No.2 2No.22
dH2O8dH2O12
total20uLtotal20uL


K204062

VectorInsert
1-8E9331
SpeI1XbaI1
PstI1PstI1
No.2 2No.22
dH2O7dH2O15
total20uLtotal20uL

K204063

VectorInsert
1-23L61-4E9
EcoRI1EcoRI1
XbaI1SpeI1
No.22No.22
dH2O10dH2O7
total20uLtotal20uL



37°C, O/N


7.min prep

sample       conc.
   X1            261.1 ng/uL
   X2           301.5
   X3           345.9
   X4           360
   X5           192
   X6           219
   X7           319
   X8          353
   X4          334

back to NOTES