August/8 October 2009

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1.sequence check
Today we conducted PCR in order to sequence the parts we have built over the summer. Using the chain-termination method and standard sequencing primers from the Parts Registry, we obtained chain-terminated clones of each sample and put them through an automatic DNA sequencer. The results will be picked up and analyzed tomorrow.

2.colony check

sample        no. of colony
 2-6O                +++
  11                   +++ 
  20                   ++
  21                   +++
 1-15L               +++
  1-15J              +++
   52                    20
   67                     2
   68                     0
   69                     0


3.ligation

sample 
 70 , 71 , 74 , 75

4.digation


K204059

VectorInsert
1-8E61-8K1
SpeI1XbaI1
PstI1PstI1
No.2 2No.22
dH2O8dH2O13
total20uLtotal20uL


K204072

VectorInsert
2-8E2316
EcoRI1EcoRI1
XbaI1SpeI1
No.22No.22
dH2O12dH2O8
total20uLtotal20uL



37 degree , 3hr
gel cut & ligation

5.transformation

sample 
70 , 71 , 74 , 75 , X4 , X4


6.color intensity check

plac+color , ptet+color

7.sequence check

sample 
22 , 47 , 40 , 25 , 51 , 50 , 31 , 32


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