Duke University/4 June 2009

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June 4, 2009

E: Gel to check Bioplastics #1-5
R: All correct sized bands
C: Use one as template for PCR

E: PCR with Bioplastics #5 as template

5x HF buffer 5 ul 
dNTPs 2 ul
forward primer 1.25 ul
reverse primer 1.25 ul
DNA template (plasmid) 0.5 ul
Phusion DNA polymerase 0.3 ul
H2O 14.7 ul 
25 ul

98°C 30s 1x
98°C 15s 
55°C 30s
72°C 1m 10s 35x
72°C 5m
4°C infinity 1x

Fragment # Name Size/Length
1 pASKphaA 4.2 kb 
2 phaB 900 bp 
3 Termcat2 1.7 kb
4 phaC 1.9 kb