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EPF-Lausanne/14 July 2009
From 2009.igem.org
Wet Lab
The digestion of the [http://partsregistry.org/Part:BBa_B0010 Terminator (BBa_B0010)] was done, in order to purify it once more, using gel extraction.
Miniprep of LacI-RBS and Inverter TetR, and PCR of the two of them.
The linearized [http://partsregistry.org/Part:BBa_B0010 Terminator (BBa_B0010)] was runned on an agarose gel and purified with a gel extraction kit.
Even though linearized [http://partsregistry.org/Part:BBa_B0010 Terminator (BBa_B0010)] concentration was very low, we tried to ligate the previously amplified LOVTAP (08.07.09) in linearized [http://partsregistry.org/Part:BBa_B0010 Terminator (BBa_B0010)]
Cloning Strategy
Primers designed for LOVTAP read-out and RBphP project.
People in the lab
- Heidi, Tu, Nath, Caro
