Modelling

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To find

PDB files of LOV domain, TrpR repressor => http://www.rcsb.org/pdb/home/home.do

To do


- Set up day of starting modeling, do a planning for modeling => see with Matteo Dal Peraro ----> will be setted up as soon as the exams are over
- Model allosteric interactions between LOVTAP & TrpR: What will be done:

-> model of LOVTAP in dark phase
-> model of LOVTAP in light phase
-> characterize how the J-alpha helix changes


- Model the fusion of LOV domain with other transcription factors


- Do a mutational assay to change specificity of LOVTAP:

Direct mutational assay: Insert mutation in specific sites thanks to the known structure of LOVTAP (already modeled on computer)
Indirect mutational assay: Random mutations, then selection of the "right" protein according to a set of selected conditions

To envisage


- Molecular mutationnal assay


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