R-BphP

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(Difference between revisions)
(The BphP / PpsR system)
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PpsR2 is a transcriptional repressor, regulated by BphP.
PpsR2 is a transcriptional repressor, regulated by BphP.
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PpsR1 and PpsR2 bind to TGTN_12ACA possibly arranged in tandem witha 7 base spacer. The affinity for both PpsRs are around 100nM.
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PpsR1 and PpsR2 bind to TGTN_12ACA possibly arranged in tandem with a 7 base spacer. The affinity for both PpsRs are around 100nM.
BphP (or BrBphP for the Brayrhizobium variant) is sensitive to far-red light (~770nm) and controls PpsR1.
BphP (or BrBphP for the Brayrhizobium variant) is sensitive to far-red light (~770nm) and controls PpsR1.
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==To find==
==To find==

Revision as of 11:25, 8 June 2009

Contents

The BphP / PpsR system

PpsR1 is a redox sensitive activator. It binds DNA under anaerobic conditions, and forms a tetramer via a disulfide bond. This interaction is ablated in the mutant PpsR1-C429S; meaning that we should be able to mimic an anaerobic system even under aerobic conditions.

PpsR2 is a transcriptional repressor, regulated by BphP.

PpsR1 and PpsR2 bind to TGTN_12ACA possibly arranged in tandem with a 7 base spacer. The affinity for both PpsRs are around 100nM.

BphP (or BrBphP for the Brayrhizobium variant) is sensitive to far-red light (~770nm) and controls PpsR1.

To find

- Define genetic circuit
- Look for exact wavelength for the corresponding light receptor Basile
- Sequences of the genes involved in the pathway (minimal genes to => [http://www.ncbi.nlm.nih.gov/ Pubmed])


To do

- Contact paper's authors

Found

- Bacterium: Bradyrhizobium can be ordered on [http://www.lgcstandards-atcc.org/LGCAdvancedCatalogueSearch/ProductDescription/tabid/1068/Default.aspx?ATCCNum=10244&Template=bacteria ATTC]


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