R-BphP

From 2009.igem.org

(Difference between revisions)
(The BphP / PpsR system)
(To do)
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==To do==
==To do==
-
;- Contact paper's authors
+
* contact Eric Giraud (giraud@mpl.ird.fr) and ask if he could send us the following material:
 +
** Bradyrhizobium ORS278
 +
** The following plasmids:
 +
*** pBAD::ppsR1
 +
*** pBAD::ppsR2
 +
** the PpsR1-C429S construct
==Found==
==Found==

Revision as of 11:36, 8 June 2009

Contents

The BphP / PpsR system

PpsR1 is a redox sensitive activator. It binds DNA under anaerobic conditions, and forms a tetramer via a disulfide bond. This interaction is ablated in the mutant PpsR1-C429S; meaning that we should be able to mimic an anaerobic system even under aerobic conditions.

PpsR2 is a transcriptional repressor, regulated by BphP.

PpsR1 and PpsR2 bind to TGTN_12ACA possibly arranged in tandem with a 7 base spacer. The affinity for both PpsRs are around 100nM.

BphP (or BrBphP for the Brayrhizobium variant) is sensitive to far-red light (~770nm) and controls PpsR1.

To find

- Define genetic circuit
- Look for exact wavelength for the corresponding light receptor Basile
- Sequences of the genes involved in the pathway (minimal genes to => [http://www.ncbi.nlm.nih.gov/ Pubmed])


To do

  • contact Eric Giraud (giraud@mpl.ird.fr) and ask if he could send us the following material:
    • Bradyrhizobium ORS278
    • The following plasmids:
      • pBAD::ppsR1
      • pBAD::ppsR2
    • the PpsR1-C429S construct

Found

- Bacterium: Bradyrhizobium can be ordered on [http://www.lgcstandards-atcc.org/LGCAdvancedCatalogueSearch/ProductDescription/tabid/1068/Default.aspx?ATCCNum=10244&Template=bacteria ATTC]


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