Team:Bologna
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- | <li><font color="#000080"><b>CIS-repressing</b></font>, to be assembled upstream of the target protein coding sequence; it contains a ribosomal binding site | + | <li><font color="#000080"><b>CIS-repressing</b></font>, to be assembled upstream of the target protein coding sequence; it contains a ribosomal binding site (RBS); |
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- | <li><font color="#000080"><b>TRANS-repressor</b></font>, to be placed under the control of a different promoter; it is complementary to the CIS-repressing, and contains a | + | <li><font color="#000080"><b>TRANS-repressor</b></font>, to be placed under the control of a different promoter; it is complementary to the CIS-repressing, and contains a RBS cover in two versions of different length (4 and 7 nucleotides). |
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= Acknowledgements = | = Acknowledgements = | ||
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Revision as of 14:38, 21 October 2009
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Project Summary
Which is our idea?
The project aims to implement a protein synthesis regulation system in Escherichia coli that acts at translational level, regardless of the target gene. This "general-purpose" device allows a faster control of protein expression.
The device was named T-Rex (Trans Repressor of Expression).
How can we achieve our goal?
T-Rex device consists of two new BioBricks:
CIS-repressing and TRANS-repressor were designed using our [[Team:Bologna/Software#1|BASER]] tool.
Transcription of the target gene produces a mRNA strand, starting with the Cis element, which is translated into proteins by ribosome. Trans’ promoter induction produces a transcript that binds with the Cis part. The RNA duplex prevents ribosome from binding to RBS, repressing protein synthesis. Thus, the TRANS-repressor amount regulates the gene mRNA translation rate.
How can we test the device?
In order to test and characterize our T-REX device, we developed the following genetic circuit:
The T-REX device is proposed as a universal and fast switch in synthetic gene circuits.
More details about our work in the Project section.
Acknowledgements
- [http://www.unibo.it/Portale/default.htm University of Bologna]
- [http://serinar.criad.unibo.it Ser.In.Ar. Cesena]
- Cultural Association San Sebastiano