Team:Bologna

From 2009.igem.org

(Difference between revisions)
(Project Summary)
Line 15: Line 15:
<html>
<html>
<font face="Calibri" font size="4" color="#000000">
<font face="Calibri" font size="4" color="#000000">
-
The project aims to implement a <b>protein synthesis regulation system</b> in <i>Escherichia coli</i> that acts at translational level, regardless of the target gene. This <b>"general-purpose"</b> device allows a faster control of protein expression.<br>The device was named <b>T-Rex</b> (<b>T</b>rans <b>R</b>epressor of <b>Ex</b>pression).
+
The project aims to design new standard parts to control the synthesis of a protein in ''Escherichia coli'' by acting at translational level regardless of the protein to be controlled. These "general-purpose" parts could allow a faster silencing of protein expression with respect to classic regulated promoters. Our device was named T-Rex (Trans Repressor of Expression). It consists of two new BioBricks, i.e. the Trans-repressor and the Cis-repressing.. This <b>"general-purpose"</b> device allows a faster control of protein expression.<br>The device was named <b>T-Rex</b> (<b>T</b>rans <b>R</b>epressor of <b>Ex</b>pression).
</font></html>
</font></html>

Revision as of 16:07, 21 October 2009

ProvaBol2.png
HOME TEAM PROJECT SOFTWARE MODELING WET LAB PARTS HUMAN PRACTICE JUDGING CRITERIA




Ely9Copia.jpg


Project Summary


Which is our idea?

The project aims to design new standard parts to control the synthesis of a protein in ''Escherichia coli'' by acting at translational level regardless of the protein to be controlled. These "general-purpose" parts could allow a faster silencing of protein expression with respect to classic regulated promoters. Our device was named T-Rex (Trans Repressor of Expression). It consists of two new BioBricks, i.e. the Trans-repressor and the Cis-repressing.. This "general-purpose" device allows a faster control of protein expression.
The device was named T-Rex (Trans Repressor of Expression).


How can we achieve our goal?

T-Rex device consists of two new BioBricks:

  • CIS-repressing, to be assembled upstream of the target protein coding sequence; it contains a ribosomal binding site (RBS);

  • TRANS-repressor, to be placed under the control of a different promoter; it is complementary to the CIS-repressing, and contains a RBS cover in two versions of different length (4 and 7 nucleotides).

CIS-repressing and TRANS-repressor were designed using our [[Team:Bologna/Software#1|BASER]] tool.

Transcription of the target gene produces a mRNA strand, starting with the Cis element, which is translated into proteins by ribosome. Trans’ promoter induction produces a transcript that binds with the Cis part. The RNA duplex prevents ribosome from binding to RBS, repressing protein synthesis. Thus, the TRANS-repressor amount regulates the gene mRNA translation rate.



Project3b.png



How can we test the device?


In order to test and characterize our T-REX device, we developed the following genetic circuit:

Circuit2.jpg



The T-REX device is proposed as a universal and fast switch in synthetic gene circuits.


More details about our work in the Project section.


Acknowledgements


  • [http://www.unibo.it/Portale/default.htm University of Bologna]


LogoUnibo.jpg








  • [http://serinar.criad.unibo.it Ser.In.Ar. Cesena]


Ser In Ar.jpg






  • Cultural Association San Sebastiano
SSebastiano.jpg











Locations of visitors to this page


Up