Revision as of 08:31, 30 July 2009

Keep track with what we did so far

This first week of wetlab we have done the following things

Transformed the plasmids with the LovTAP gene, generously sent by Dr. Sosnick's lab from Chicago universtiy, into competent E.Coli
Designed the cloning strategy for cloning the LovTAP gene from its original vector to a iGEM vector+add an inducible promoter (LacI) (+RBS +term.)
Ordered and received the primers needed for the PCR of LovTAP
Designed the cloning strategy for inclusion of the LovTAP BioBrick with different reporting cassettes
Transformed all the BioBricks that will be needed for the cloning strategies (c.f. notebook for more information about these parts) into competent E.Coli
Fused the two BioBricks "LacI" and "RBS"
Digested the LovTAP PCR products and RBS part

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