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Team:EPF-Lausanne/Notebook/Cloning Strategy
From 2009.igem.org
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=Cloning strategy= | =Cloning strategy= | ||
==July== | ==July== | ||
| - | ===06.07.09=== | + | ===Week 1=== |
| + | ====06.07.09==== | ||
Four forward primers were designed to amplify: | Four forward primers were designed to amplify: | ||
<br> 1.Promoter T7, RBS, CBP and LOVTAP: | <br> 1.Promoter T7, RBS, CBP and LOVTAP: | ||
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The '''recipient IGEM part''' have been chosen: [http://partsregistry.org/partsdb/get_part.cgi?part=BBa_B0010 BBa_B0010], well 13D in the received kit plate 1 | The '''recipient IGEM part''' have been chosen: [http://partsregistry.org/partsdb/get_part.cgi?part=BBa_B0010 BBa_B0010], well 13D in the received kit plate 1 | ||
| - | ===07.07.09=== | + | ====07.07.09==== |
To design plasmids : software Vector NTI | To design plasmids : software Vector NTI | ||
| - | ===08.07.09=== | + | ====08.07.09==== |
| - | ===09.07.09=== | + | ====09.07.09==== |
| - | ===10.07.09=== | + | ====10.07.09==== |
==August== | ==August== | ||
</div><div CLASS="epfl09bouchon"></div> | </div><div CLASS="epfl09bouchon"></div> | ||
Revision as of 14:48, 8 July 2009
Contents |
Cloning strategy
July
Week 1
06.07.09
Four forward primers were designed to amplify:
1.Promoter T7, RBS, CBP and LOVTAP:
- gtttcttcgaattcgcggccgcttctagagtaatacgactcactataggggaattgtg
2.RBS, CBP and LOVTAP:
- gtttcttcgaattcgcggccgcttctagagtgtttaactttaagaaggag
3.CBP and LOVTAP:
- gtttcttcgaattcgcggccgcttctagatgaagcgacgatggaaaaagaatttcatag
4.LOVTAP:
- gtttcttcgaattcgcggccgcttctagatgctactacacttgaacgtattgagaagaac
One reverse primer were designed:
- gtttcttcctgcagcggccgctactagtatcaatcgcttttcagcaacacctcttc
The recipient IGEM part have been chosen: [http://partsregistry.org/partsdb/get_part.cgi?part=BBa_B0010 BBa_B0010], well 13D in the received kit plate 1
07.07.09
To design plasmids : software Vector NTI
