Team:EPF-Lausanne/Notebook/Cloning Strategy

From 2009.igem.org

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(July)
(July)
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=Cloning strategy=
=Cloning strategy=
==July==
==July==
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===Week 1===
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====06.07.09====
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===06.07.09===
Four forward primers were designed to amplify:
Four forward primers were designed to amplify:
<br> 1.Promoter T7, RBS, CBP and LOVTAP:  
<br> 1.Promoter T7, RBS, CBP and LOVTAP:  
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The '''recipient IGEM part''' have been chosen: [http://partsregistry.org/partsdb/get_part.cgi?part=BBa_B0010 BBa_B0010], well 13D in the received kit plate 1
The '''recipient IGEM part''' have been chosen: [http://partsregistry.org/partsdb/get_part.cgi?part=BBa_B0010 BBa_B0010], well 13D in the received kit plate 1
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====07.07.09====
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===07.07.09===
To design plasmids : software Vector NTI
To design plasmids : software Vector NTI
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====08.07.09====
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===08.07.09===
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====09.07.09====
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===09.07.09===
====10.07.09====
====10.07.09====

Revision as of 14:48, 8 July 2009

Contents


Cloning strategy

July

06.07.09

Four forward primers were designed to amplify:
1.Promoter T7, RBS, CBP and LOVTAP:

gtttcttcgaattcgcggccgcttctagagtaatacgactcactataggggaattgtg

2.RBS, CBP and LOVTAP:

gtttcttcgaattcgcggccgcttctagagtgtttaactttaagaaggag

3.CBP and LOVTAP:

gtttcttcgaattcgcggccgcttctagatgaagcgacgatggaaaaagaatttcatag

4.LOVTAP:

gtttcttcgaattcgcggccgcttctagatgctactacacttgaacgtattgagaagaac

One reverse primer were designed:

gtttcttcctgcagcggccgctactagtatcaatcgcttttcagcaacacctcttc


The recipient IGEM part have been chosen: [http://partsregistry.org/partsdb/get_part.cgi?part=BBa_B0010 BBa_B0010], well 13D in the received kit plate 1

07.07.09

To design plasmids : software Vector NTI

08.07.09

09.07.09

10.07.09

August