Team:EPF-Lausanne/Notebook/Cloning Strategy

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{{EPF-Lausanne09}}
 
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<div CLASS="epfltrick">__TOC__
 
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</div><div CLASS="epfl09">
 
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=Cloning strategy=
 
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==06.07.09==
 
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Four forward primers were designed to amplify:
 
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<br> 1.Promoter T7, RBS, CBP and LOVTAP:
 
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:gtttcttcgaattcgcggccgcttctagagtaatacgactcactataggggaattgtg
 
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2.RBS, CBP and LOVTAP:
 
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:gtttcttcgaattcgcggccgcttctagagtgtttaactttaagaaggag
 
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3.CBP and LOVTAP:
 
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:gtttcttcgaattcgcggccgcttctagatgaagcgacgatggaaaaagaatttcatag
 
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4.LOVTAP:
 
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:gtttcttcgaattcgcggccgcttctagatgctactacacttgaacgtattgagaagaac
 
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One reverse primer were designed:
 
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:gtttcttcctgcagcggccgctactagtatcaatcgcttttcagcaacacctcttc
 
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The '''recipient IGEM part''' have been chosen: [http://partsregistry.org/partsdb/get_part.cgi?part=BBa_B0010 BBa_B0010], well 13D in the received kit plate 1
 
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==07.07.09==
 
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==08.07.09==
 
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</div><div CLASS="epfl09bouchon"></div>
 

Latest revision as of 08:05, 28 July 2009