Team:Groningen/Future

From 2009.igem.org

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Ideas for future work ({{todo}} references):
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==Labwork==
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===Metals===
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A large amount of progress has been obtained in the research line of arsenic accumulation, however several other interesting metals (e.g. copper, zinc) had to be abandoned which might provide more more interesting applications. For this to occur the constructs that were abandoned along the way (e.g. SmtA, HmtA) should be finished and characterized. But also for the existing arsenic devices more characterizing can be performed, for example more detailed characterization of the uptake capacity of the transporter. In this project fermentation was used to supply the culture with medium saturated with air in higher amount than can be attained in shake cultures. This was to ensure a higher amount of gas to be available to enter the gas vesicles, for a future project it might be of interest to sparge nitrogen into broth to obtain anaerobic conditions. Anaerobic condition will influence the oxidation state of arsenic, probably having an effect on the uptake efficiency.
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* Reusing the bacteria by moving them to another container and using Sb to accelerate As efflux ([[Team:Groningen/Literature#Meng2004|Meng2004]]).
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===Gas vesicles===
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* Demonstrating the presence of arsenic by putting Luciferase under regulation of ArsR.
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A nice addition to the gas vesicle cluster would be the removal of the 10 time repeat, which was attempted in our project without succes.
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* Use hydrophobic chaplin proteins to keep the bacteria floating on the surface of the medium (it won't help them to start floating)
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** This might be used to start bouyancy with GVP and keep them on the surface of the medium with these proteins. These proteins origin from Gram positive bacteria, which may cause problems. Information may be asked from: Dennis Klaassen (MolMic).
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*[[Safety]]
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*[[Summer events]] like the Kluyver day?
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* Using the ability of the roots of some plants (unknown which exactly have this feature) to solubilize metal ions from solid metal in combination with our bacterium could lead to improved detoxification of the environment as also peaces of copper or zinc waste etc.
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==Labwork==
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*Removing repeat of GVP
*Removing repeat of GVP
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*Completing SmtA
 
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*Completing HmtA
 
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*Characterize the uptake capacity of transporters
 
*Nitrogen sparging in fermentation to see the effect of oxidation states of heavy metals
*Nitrogen sparging in fermentation to see the effect of oxidation states of heavy metals
*Use higher concentrations in death assays
*Use higher concentrations in death assays

Revision as of 12:58, 21 October 2009

[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]

Labwork

Metals

A large amount of progress has been obtained in the research line of arsenic accumulation, however several other interesting metals (e.g. copper, zinc) had to be abandoned which might provide more more interesting applications. For this to occur the constructs that were abandoned along the way (e.g. SmtA, HmtA) should be finished and characterized. But also for the existing arsenic devices more characterizing can be performed, for example more detailed characterization of the uptake capacity of the transporter. In this project fermentation was used to supply the culture with medium saturated with air in higher amount than can be attained in shake cultures. This was to ensure a higher amount of gas to be available to enter the gas vesicles, for a future project it might be of interest to sparge nitrogen into broth to obtain anaerobic conditions. Anaerobic condition will influence the oxidation state of arsenic, probably having an effect on the uptake efficiency.

Gas vesicles

A nice addition to the gas vesicle cluster would be the removal of the 10 time repeat, which was attempted in our project without succes.

  • Removing repeat of GVP
  • Nitrogen sparging in fermentation to see the effect of oxidation states of heavy metals
  • Use higher concentrations in death assays

Modeling