Team:Groningen/Notebook/15 September 2009

From 2009.igem.org

(Difference between revisions)
(New page: {{Team:Groningen/Notebook/Day/Header}} ==Wet== ===GVP Cluster=== '''Planning''' :→ {{todo}} work out the wiki page for GVP :→ {{todo}} make a doodle for presentation planning (1-19...)
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'''Plates'''
 +
 +
The
 +
 +
{|cellpadding="2" cellspacing="1" border="4"
 +
|'''Name'''
 +
|'''Plasmid Used'''
 +
|'''Antibiotics on Plasmid''' 
 +
|'''No. of Colonies'''
 +
|'''Date'''
 +
|-
 +
|GlpF no.1 (low)
 +
|pSB1AC3
 +
|Ampicillin
 +
|0
 +
|14 sept.
 +
|-
 +
|GlpF no.1 (high)
 +
|pSB1AC3
 +
|Ampicillin
 +
|2
 +
|14 sept.
 +
|-
 +
|GlpF no.2 (low)
 +
|pSB1AC3
 +
|Ampicillin
 +
|1
 +
|14 sept.
 +
|-
 +
|GlpF no.2 (high)
 +
|pSB1AC3
 +
|Ampicillin
 +
|1
 +
|14 sept.
 +
|-
 +
|pLacI (low)
 +
|pSB1AC3
 +
|Ampicillin
 +
|~30
 +
|14 sept.
 +
|-
 +
|pLacI (high)
 +
|pSB1AC3
 +
|Ampicillin
 +
|~80
 +
|14 sept.
 +
|-
 +
|pLacI-GVP no.1 (low)
 +
|pSB1A2
 +
|Ampicillin
 +
|0
 +
|14 sept.
 +
|-
 +
|pLacI-GVP no.1 (high)
 +
|pSB1A2
 +
|Ampicillin
 +
|4
 +
|14 sept.
 +
|-
 +
|pLacI-GVP no.2 (low)
 +
|pSB1A2
 +
|Ampicillin
 +
|0
 +
|14 sept.
 +
|-
 +
|pLacI-GVP no.2 (high)
 +
|pSB1A2
 +
|Ampicillin
 +
|9
 +
|14 sept.
 +
|-
 +
|Positive (J23101) (high)
 +
|J61002
 +
|Ampicillin
 +
|~1500
 +
|14 sept.
 +
|-
 +
|Negative (MQ) (high)
 +
|MQ
 +
|Ampicillin
 +
|0
 +
|14 sept.
 +
|}
 +
 +
 +
:→ The plates showed a low amount of colonies, and also red in the case of the positive plate.
 +
 +
 +
:→ The two plates with stripes of pZntR-GVP (pSB2K3) and pCueO-GVP (pSB2K3) culture for glycerol stocks showed growth and some single colonies.
 +
 +
 +
:→ All plates were stored in the fridge for further use.
 +
 +
 +
'''Cultures'''
 +
 +
The overnight cultures with LB-amp<sub>100</sub> medium of colonies E.coli TOP10 with
===Transporters===
===Transporters===

Revision as of 08:38, 15 September 2009

Igemhomelogo.png

Wet

GVP Cluster

Planning

TODO work out the wiki page for GVP
TODO make a doodle for presentation planning (1-19 oct.)
TODO media attention
TODO


Plates

The

Name Plasmid Used Antibiotics on Plasmid No. of Colonies Date
GlpF no.1 (low) pSB1AC3 Ampicillin 0 14 sept.
GlpF no.1 (high) pSB1AC3 Ampicillin 2 14 sept.
GlpF no.2 (low) pSB1AC3 Ampicillin 1 14 sept.
GlpF no.2 (high) pSB1AC3 Ampicillin 1 14 sept.
pLacI (low) pSB1AC3 Ampicillin ~30 14 sept.
pLacI (high) pSB1AC3 Ampicillin ~80 14 sept.
pLacI-GVP no.1 (low) pSB1A2 Ampicillin 0 14 sept.
pLacI-GVP no.1 (high) pSB1A2 Ampicillin 4 14 sept.
pLacI-GVP no.2 (low) pSB1A2 Ampicillin 0 14 sept.
pLacI-GVP no.2 (high) pSB1A2 Ampicillin 9 14 sept.
Positive (J23101) (high) J61002 Ampicillin ~1500 14 sept.
Negative (MQ) (high) MQ Ampicillin 0 14 sept.


→ The plates showed a low amount of colonies, and also red in the case of the positive plate.


→ The two plates with stripes of pZntR-GVP (pSB2K3) and pCueO-GVP (pSB2K3) culture for glycerol stocks showed growth and some single colonies.


→ All plates were stored in the fridge for further use.


Cultures

The overnight cultures with LB-amp100 medium of colonies E.coli TOP10 with

Transporters

Metal Accumulation

Vectors

Dry

April
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30