Team:Groningen/Notebook/15 September 2009
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Revision as of 08:38, 15 September 2009 by Verhoeven1981 (Talk | contribs)
Wet
GVP Cluster
Planning
- → TODO work out the wiki page for GVP
- → TODO make a doodle for presentation planning (1-19 oct.)
- → TODO media attention
- → TODO
Plates
The
Name | Plasmid Used | Antibiotics on Plasmid | No. of Colonies | Date |
GlpF no.1 (low) | pSB1AC3 | Ampicillin | 0 | 14 sept. |
GlpF no.1 (high) | pSB1AC3 | Ampicillin | 2 | 14 sept. |
GlpF no.2 (low) | pSB1AC3 | Ampicillin | 1 | 14 sept. |
GlpF no.2 (high) | pSB1AC3 | Ampicillin | 1 | 14 sept. |
pLacI (low) | pSB1AC3 | Ampicillin | ~30 | 14 sept. |
pLacI (high) | pSB1AC3 | Ampicillin | ~80 | 14 sept. |
pLacI-GVP no.1 (low) | pSB1A2 | Ampicillin | 0 | 14 sept. |
pLacI-GVP no.1 (high) | pSB1A2 | Ampicillin | 4 | 14 sept. |
pLacI-GVP no.2 (low) | pSB1A2 | Ampicillin | 0 | 14 sept. |
pLacI-GVP no.2 (high) | pSB1A2 | Ampicillin | 9 | 14 sept. |
Positive (J23101) (high) | J61002 | Ampicillin | ~1500 | 14 sept. |
Negative (MQ) (high) | MQ | Ampicillin | 0 | 14 sept. |
- → The plates showed a low amount of colonies, and also red in the case of the positive plate.
- → The two plates with stripes of pZntR-GVP (pSB2K3) and pCueO-GVP (pSB2K3) culture for glycerol stocks showed growth and some single colonies.
- → All plates were stored in the fridge for further use.
Cultures
The overnight cultures with LB-amp100 medium of colonies E.coli TOP10 with
Transporters
Metal Accumulation
Vectors
Dry
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