Team:Groningen/Notebook/19 June 2009
From 2009.igem.org
- All o/n cultures were succesfull.
- 30% glycerol stocks were made, the stocks were flash frozen before they were put in -80dg freezer. (labeled with BBa_nr, gene nr1-3 and date)
- DNA was isolated from them, using the Nucleospin plasmid isolation kit.
- Concentration of the samples were:
- ??
- ??
- ??
- Concentration of the samples were:
- 1ug of the gvp containing plasmids were digested with EcoRI and XbaI in 2x buffer Tango, for 1hr @ 37dg.
- These samples and the uncut plasmids containing gvp, RBS and Term were loaded on an Agarose gel.
- For gvp/EcoRI-XbaI a band of 1.4kb and >5kb was found. This correlates to the expected size.
- For the other plasmids several bands were seen.
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